Cloning, sequencing, and expression of nitrile hydratase gene of mutant 4D strain of Rhodococcus rhodochrous PA 34 in E. coli.

Abstract

The NHase encoding gene of mutant 4D was isolated by PCR amplification. The NHase gene of mutant 4D was successfully cloned and expressed in Escherichia coli by using Ek/LIC Duet cloning kits (Novagen). For the active expression of the NHase gene, the co-expression of small cobalt transporter gene (P-protein gene) has also been co-expressed with NHase gene… (More)
DOI: 10.1007/s12010-012-9790-9

Topics

Cite this paper

@article{Pratush2012CloningSA, title={Cloning, sequencing, and expression of nitrile hydratase gene of mutant 4D strain of Rhodococcus rhodochrous PA 34 in E. coli.}, author={Amit Pratush and Amit Seth and Tek Chand Bhalla}, journal={Applied biochemistry and biotechnology}, year={2012}, volume={168 3}, pages={465-86} }