Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis.

Abstract

The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution.

Cite this paper

@article{Bond2000CloningSA, title={Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis.}, author={Chris J. Bond and Jing Yan Huang and Roman Hajduk and Karen E. Flick and Peter J Heath and Barry L. Stoddard}, journal={Acta crystallographica. Section D, Biological crystallography}, year={2000}, volume={56 Pt 11}, pages={1359-66} }