Cloning of the riboB locus of Aspergillus nidulans.

  title={Cloning of the riboB locus of Aspergillus nidulans.},
  author={C. Elizabeth Oakley and Clifford F. Weil and Patricia L Kretz and Berl R Oakley},
  volume={53 2-3},
We have complemented the riboB2 mutation of Aspergillus nidulans by transformation with a plasmid library of wild-type (wt) sequences. We have isolated, by marker rescue from a riboB+ transformant, a plasmid that complements riboB2 efficiently. From this plasmid we have subcloned an A. nidulans sequence that complements riboB2 efficiently and that integrates by homologous recombination at a site closely linked to the riboB locus. We conclude that this sequence contains the wt riboB+ allele. 

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