Cloning of the replication gene P of bacteriophage lambda: Effects of increased P-protein synthesis on cellular and phage DNA replication

@article{Klinkert1979CloningOT,
  title={Cloning of the replication gene P of bacteriophage lambda: Effects of increased P-protein synthesis on cellular and phage DNA replication},
  author={J Klinkert and Albrecht W. Klein},
  journal={Molecular and General Genetics MGG},
  year={1979},
  volume={171},
  pages={219-227}
}
SummaryA restriction fragment of λDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/λE complement λPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/glE such that induction of the lac promoter by IPTG or lactose leads to increased production of the P gene product. A high amount of P protein in E. coli cells results in a slow inhibition of bacterial DNA… CONTINUE READING

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