Cloning of the kanamycin resistance gene from a kanamycin-producing Streptomyces species.

@article{Nakano1984CloningOT,
  title={Cloning of the kanamycin resistance gene from a kanamycin-producing Streptomyces species.},
  author={Michiko M. Nakano and Hiroshi Mashiko and Hiroshi Ogawara},
  journal={Journal of bacteriology},
  year={1984},
  volume={157 1},
  pages={79-83}
}
A kanamycin-producing strain, Streptomyces kanamyceticus ISP5500, is resistant to kanamycin. A kanamycin resistance determinant was cloned from S. kanamyceticus into Streptomyces lividans 1326, using the plasmid vector pIJ702. The resulting plasmid, pMCP5, could also transform Streptomyces lavendulae S985 and Streptomyces parvulus 2283 to kanamycin resistance. Transformants carrying pMCP5 were markedly more resistant than S. kanamyceticus to the aminoglycoside antibiotics sisomicin, tobramycin… CONTINUE READING
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from Streptomyces and their use in vector construction

  • R. H. Skinner, J. Thompson, J. M. Ward, E. Cundliffe
  • 1982

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