Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1

  title={Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1},
  author={Roger K. Sunahara and H C Guan and Brian F O'dowd and Philip Seeman and Lisanne G. Laurier and Gordon Y. K. Ng and Susan R. George and J Torchia and Hubert H.M. Van Tol and Hyman B. Niznik},
DOPAMINE receptors belong to a superfamily of receptors that exert their biological effects through guanine nucleotide-binding (G) proteins. Two main dopamine receptor subtypes have been identified, D1 and D2, which differ in their pharmacological and biochemical characteristics. D1 stimulates adenylyl cyclase activity, whereas D2 inhibits it1–3. Both receptors are primary targets for drugs used to treat many psychomotor diseases, including Parkinson's disease and schizophrenia4,5. Whereas the… 

Coupling of D1 and D5 dopamine receptors to multiple G proteins

  • A. Sidhu
  • Biology
    Molecular Neurobiology
  • 2007
Dopamine receptors are a subclass of the super family of G protein-coupled receptors, that transduce their effects by coupling to specific G proteins. Within the dopamine receptor family, the

Dopamine receptors: from structure to function.

Target deletion of several of these dopamine receptor genes in mice should provide valuable information about their physiological functions and provide unequivocal evidence for the involvement of one of these receptors in the etiology of various central nervous system disorders.

Dopamine Receptors and Antipsychotic Drug Response

This review summarises what is currently understood about dopamine receptors, their role in antipsychotic drug action, and their association with psychosis.

Dopamine receptor genes: new tools for molecular psychiatry.

Recent work on the cloning and characterization of some of the members of the dopamine receptor gene family (D1, D2, D4, D5), their relationship to neuropsychiatric disorders and their potential role in antipsychotic drug action is reviewed.

The Dopamine D1D Receptor

The isolation of four distinct vertebrate dopamine D1 receptor subtypes suggests the existence of additional mammalian D1 like receptor genes that may account for the observed pharmacological and biochemical multiplicity of dopamine D 1-like receptor mediated events.

Characterization of a chimeric human dopamine D3/D2 receptor functionally coupled to adenylyl cyclase in Chinese hamster ovary cells.

The ability of D3 ligand binding domains to produce a D2 functional response implies that the third intracellular loop of the D3 receptor is unable to mediate this D2 response in Chinese hamster ovary cells.

The renal dopamine receptors.

Dopamine is an endogenous catecholamine that modulates many functions including behavior, movement, nerve conduction, hormone synthesis and release, blood pressure, and ion fluxes and has been postulated to act as an intrarenal natriuretic hormone.

New insights into dopamine receptors in the central nervous system

  • P. Strange
  • Biology, Chemistry
    Neurochemistry International
  • 1993

Molecular neurobiology of dopaminergic receptors.




Cloning and expression of a rat D2 dopamine receptor cDNA

This receptor has been characterized on the basis of three criteria: the deduced amino-acid sequence which reveals that it is a member of the family of G-protein-coupled receptors; the tissue distribution of the mRNA which parallels that of the D2 dopamine receptor; and the pharmacological profile of mouse fibroblast cells transfected with the cDNA.

Human dopamine D1 receptor encoded by an intronless gene on chromosome 5

The cloning of the D1 receptor gene is reported, which resides on an intronless region on the long arm of chromosome 5, near two other members of the G-linked receptor family, and binds drugs with affinities identical to the native human D1 receptors.

Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine

The cloning of a gene that encodes a dopamine receptor gene that has high homology to the human dopamine D2 and D3 receptor genes is reported, which suggests the existence of other types of dopamine receptors which are more sensitive to clozapine.

Molecular cloning and expression of the gene for a human D1 dopamine receptor

Transient or stable expression of the cloned gene in host cells established specific ligand binding and functional activity characteristic of a D1 dopamine receptor coupled to stimulation of adenylyl cyclase.

Cloning and expression of human and rat Dt dopamine receptors

The cloning of human and rat D1 dopamine receptors by applying an approach based on the polymerase chain reaction is reported, which reveals that it is a G protein-coupled receptor.

Link between D1 and D2 dopamine receptors is reduced in schizophrenia and Huntington diseased brain.

The D1-D2 link may be mediated by guanine nucleotide-binding protein components influencing behavior, since the link was missing in over half the postmortem striata from patients with schizophrenia and Huntington disease but was present in human control, Alzheimer, and Parkinson striata.

Review: D1 dopamine receptor—the search for a function: A critical evaluation of the D1/D2 dopamine receptor classification and its functional implications

A variety of biochemical, behavioral, and electrophysiological evidence is presented which supports the view that D1 and D2 DA receptors can interact in both an opposing and synergistic fashion.

Molecular cloning and expression of a D1 dopamine receptor linked to adenylyl cyclase activation.

In order to clone the D1 dopamine receptor linked to adenylyl cyclase activation, the polymerase chain reaction was used with highly degenerate primers to selectively amplify a cDNA sequence from NS20Y neuroblastoma cell mRNA, exhibiting considerable sequence homology to guanine nucleotide-binding (G)-protein-coupled receptors that have been cloned previously.

The major dopamine D2 receptor: molecular analysis of the human D2A subtype.

The structural diversity of the human D2 dopamine receptor was examined at the nucleic acid level. Sequence analysis of receptor cDNA clones isolated from human brain and pituitary libraries and