Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli.

@article{Bohach1988CloningOT,
  title={Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli.},
  author={Gregory A. Bohach and Alan R Hauser and Patrick Schlievert},
  journal={Infection and immunity},
  year={1988},
  volume={56 6},
  pages={1665-7}
}
The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable… CONTINUE READING

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