Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza A viruses in avian cells.

@article{Massin2005CloningOT,
  title={Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza A viruses in avian cells.},
  author={Pascale Massin and Pierre Rodrigues and Monica Marasescu and Sylvie van der Werf and Nadia Naffakh},
  journal={Journal of virology},
  year={2005},
  volume={79 21},
  pages={13811-6}
}
Reverse genetics techniques to rescue influenza viruses have thus far been based on the use of a human polymerase I (PolI) promoter to direct the synthesis of the eight viral RNAs. They can only be used on cells from primate origin due to the species specificity of the PolI promoter. Here we report the cloning of the chicken PolI promoter sequence and the generation of recombinant influenza virus upon transfection of bidirectional PolI/PolII plasmids in avian cells. Potential contributions of… CONTINUE READING

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