Cloning of the V-ATPase subunit G in plant: functional expression and sub-cellular localization.

@article{Rouqui1998CloningOT,
  title={Cloning of the V-ATPase subunit G in plant: functional expression and sub-cellular localization.},
  author={David Rouqui{\'e} and Colette Tournaire-Roux and Wojciech Szponarski and Michel Rossignol and Patrick Doumas},
  journal={FEBS letters},
  year={1998},
  volume={437 3},
  pages={287-92}
}
A 13-kDa tobacco plasma membrane protein was isolated from two-dimensional electrophoresis gels. After microsequencing, RT-PCR techniques and cDNA library screening allowed for the cloning of two cDNAs. These cDNAs encoded for the subunit G of the vacuolar H+-ATPase, the first one identified in plants. Analysis of mRNA distribution showed a maximum level in the leaves and in the stem of the apical part of the tobacco plant. Heterologous functional complementation of the yeast mutant (deltavma10… CONTINUE READING

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