Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae.

@article{Dohmen1990CloningOT,
  title={Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae.},
  author={R. J{\"u}rgen Dohmen and Alexander W. M. Strasser and Ulrike M. Dahlems and Cornelis P. Hollenberg},
  journal={Gene},
  year={1990},
  volume={95 1},
  pages={111-21}
}
The Schwanniomyces occidentalis glucoamylase (GAM)-encoding gene (GAM1) was isolated from a lambda Charon4A genomic library using synthetic oligodeoxynucleotides as probes. GAM1 contains an ORF of 2874 nucleotides (nt) coding for 958 amino acids. S1 mapping revealed that the transcript has only a very short 5'-untranslated leader of 8-12 nt. Disruption and displacement of the GAM1 gene in Sc. occidentalis resulted in loss of the ability to grow on starch efficiently. The gam1 strains still… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.
14 Extracted Citations
0 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 14 extracted citations

Similar Papers

Loading similar papers…