Cloning of the Saccharomyces cerevisiae gene whose overexpression overcomes the effects of HM-1 killer toxin, which inhibits beta-glucan synthesis.

@article{Kasahara1994CloningOT,
  title={Cloning of the Saccharomyces cerevisiae gene whose overexpression overcomes the effects of HM-1 killer toxin, which inhibits beta-glucan synthesis.},
  author={Shin Kasahara and Hiroshi Yamada and Toshiyuki Mio and Yasuhiko Shiratori and Chizuko Miyamoto and Tomio Yabe and Tasuku Nakajima and Eiji Ichishima and Yasuhiro Furuichi},
  journal={Journal of bacteriology},
  year={1994},
  volume={176 5},
  pages={1488-99}
}
A gene whose overexpression can endow Saccharomyces cerevisiae cells with resistance to HM-1 killer toxin was cloned from an S. cerevisiae genomic library. This gene, designated HKR1 (Hansenula mrakii killer toxin-resistant gene 1), contains a 5.4-kb open reading frame. The predicted amino acid sequence of the protein specified by HKR1 indicates that the protein consists of 1,802 amino acids and is very rich in serine and threonine, which could serve as O-glycosylation sites. The protein also… CONTINUE READING