Cloning of the RNase H genes from a metagenomic DNA library: identification of a new type 1 RNase H without a typical active-site motif.

Abstract

AIMS The study aimed to combine a metagenomics approach with complementary genetics to identify novel bacterial genes with orthologous functions, with the identification of novel RNase H genes as a test case. METHODS AND RESULTS A metagenomic DNA library was prepared from leaf-and-branch compost and used to screen for the RNase H genes by their abilities… (More)
DOI: 10.1111/j.1365-2672.2010.04724.x

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Cite this paper

@article{Kanaya2010CloningOT, title={Cloning of the RNase H genes from a metagenomic DNA library: identification of a new type 1 RNase H without a typical active-site motif.}, author={Eiko Kanaya and T. Sakabe and N. T. Nguyen and Satoshi Koikeda and Y. Koga and Kazufumi Takano and Seiichiro Kanaya}, journal={Journal of applied microbiology}, year={2010}, volume={109 3}, pages={974-83} }