Cloning of synthetic deoxyribonucleic acid that codes for embryonic cardiac myosin light-chain polypeptide.


Double-stranded complementary deoxyribonucleic acid (cDNA) transcribed in vitro from a partially pure myosin light-chain messenger ribonucleic acid (mRNA) of the chick embryonic heart was cloned in Escherichia coli strain chi 1776 by using the HindIII cleavage site in the plasmid pBR322. The insertion of essentially full length DNA was achieved by repeated… (More)


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