Cloning of sucrose:sucrose 1-fructosyltransferase from onion and synthesis of structurally defined fructan molecules from sucrose.

@article{Vijn1998CloningOS,
  title={Cloning of sucrose:sucrose 1-fructosyltransferase from onion and synthesis of structurally defined fructan molecules from sucrose.},
  author={Irma Vijn and Anja J H van Dijken and Matthias L{\"u}scher and Anja Bos and E. H. J. Smeets and Peter Weisbeek and Andres Wiemken and Sjef Cm Smeekens},
  journal={Plant physiology},
  year={1998},
  volume={117 4},
  pages={1507-13}
}
Sucrose (Suc):Suc 1-fructosyltransferase (1-SST) is the key enzyme in plant fructan biosynthesis, since it catalyzes de novo fructan synthesis from Suc. We have cloned 1-SST from onion (Allium cepa) by screening a cDNA library using acid invertase from tulip (Tulipa gesneriana) as a probe. Expression assays in tobacco (Nicotiana plumbaginifolia) protoplasts showed the formation of 1-kestose from Suc. In addition, an onion acid invertase clone was isolated from the same cDNA library. Protein… CONTINUE READING
29 Citations
22 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 29 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 22 references

Cloning and sequencing of the 1 - SST cDNA from chicory root

  • S Halleux, P Cutsem
  • Plant Physiol
  • 1997

Purification and characterization of 1 - SST , the key enzyme initiating fructan biosynthesis in young chicory roots ( Cichorium intybus )

  • W Vandenende, D Wonterghem, E Dewil, P Verheart, A Loof, A Laere
  • Physiol Plant
  • 1996

Fructan : an extension of sucrose by sucrose

  • A Wiemken, N Sprenger, T Boller
  • 1995
1 Excerpt

Purification and characterization of fructans with b - 2 , 1 - and b - 2 , 6 - glycosidic linkages suitable for enzyme studies

  • GD Bonnett, IM Sims, John JAS, RJ Simpson
  • New Phytol
  • 1994
1 Excerpt

Similar Papers

Loading similar papers…