• Biology, Medicine
  • Published in Journal of bacteriology 1980

Cloning of replication, incompatibility, and stability functions of R plasmid NR1.

@article{Miki1980CloningOR,
  title={Cloning of replication, incompatibility, and stability functions of R plasmid NR1.},
  author={Takeyoshi Miki and Alan Easton and R. H. Rownd},
  journal={Journal of bacteriology},
  year={1980},
  volume={141 1},
  pages={
          87-99
        }
}
The region of R plasmid NR1 that is capable of mediating autonomous replication was cloned by using EcoRI, SalI, and PstI restriction endonucleases. The only EcoRI fragment capable of mediating autonomous replication in either a pol+ or a polA host was fragment B. SalI fragment E joined in native orientation with the part of SalI fragment C that overlapped with EcoRI fragment B, and also two contiguous PstI fragments of sizes 1.6 and 1.1 kilobases from EcoRI fragment B-mediated autonomous… CONTINUE READING

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