Cloning of rat Sertoli cell follicle-stimulating hormone primary response complementary deoxyribonucleic acid: regulation of TSC-22 gene expression.

@article{Hamil1994CloningOR,
  title={Cloning of rat Sertoli cell follicle-stimulating hormone primary response complementary deoxyribonucleic acid: regulation of TSC-22 gene expression.},
  author={Katherine G Hamil and Susan H. Hall},
  journal={Endocrinology},
  year={1994},
  volume={134 3},
  pages={1205-12}
}
The molecular mechanisms underlying the pleiotropic effects of FSH were investigated by screening a plasmid cDNA library for clones hybridizing to FSH-regulated RNAs. Recombinant colonies were selected at random, and plasmids were purified, radiolabeled, and hybridized to Northern blots containing RNA extracted from control and FSH-treated Sertoli cells. Of 210 clones screened by this method, 10 hybridized to transcripts that were regulated either positively or negatively by FSH. DNA sequence… CONTINUE READING

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