Cloning of penaeidin gene promoter in tiger shrimp (Penaeus monodon).

  title={Cloning of penaeidin gene promoter in tiger shrimp (Penaeus monodon).},
  author={Shih-Hu Ho and Y L Song},
  journal={Fish & shellfish immunology},
  volume={27 1},
Penaeidins belong to a family of antimicrobial peptides that are expressed in the hemocytes of penaeid shrimps. Using an extender PCR method and a nested PCR, we cloned two types of genomic fragment flanking the 5' end of penaeidin gene in tiger shrimp (Penaeus monodon): Type536 and Type411 sequences. Both fragments contained TATA box, GATA, dorsal and AP-1 motifs and were ligated to an expression vector with a luciferase reporter gene. The constructs were then delivered into Drosophila S2 cell… CONTINUE READING


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