Cloning of murine SeGpx cDNA and synthesis of mutated GPx proteins in Escherichia coli.

Abstract

Glutathione peroxidase (GPx) of mammalian cells and Escherichia coli formate dehydrogenase both contain a selenocysteine (SeCys) in their amino acid (aa) sequence. In these two enzymes, this aa is encoded by a UGA codon, which is usually a stop codon for protein synthesis. We constructed plasmids to test the synthesis of GPx in E. coli. These constructions… (More)

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@article{Rocher1991CloningOM, title={Cloning of murine SeGpx cDNA and synthesis of mutated GPx proteins in Escherichia coli.}, author={Christophe Rocher and Chi Faucheu and Fabienne Herv{\'e} and Claude B{\'e}nicourt and Jennifer Lalanne}, journal={Gene}, year={1991}, volume={98 2}, pages={193-200} }