Cloning of genomic DNA of Rhizopus niveus lipase and expression in the yeast Saccharomyces cerevisiae.

@article{Kohno1998CloningOG,
  title={Cloning of genomic DNA of Rhizopus niveus lipase and expression in the yeast Saccharomyces cerevisiae.},
  author={Mitsutaka Kohno and M Enatsu and Wataru Kugimiya},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1998},
  volume={62 12},
  pages={2425-7}
}
Genomic DNA encoding Lipase I was cloned from Rhizopus niveus strain IFO4759. For expression of this gene in S. cerevisiae, DBY746 was transformed with YEp352PLipS, which had the cloned lipase gene under the control of a PGK promoter. This strain secreted the lipase at a high level (350 U/ml). The strain ND-12B was produced by a mating of DBY746, harboring… CONTINUE READING