Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II

  title={Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II},
  author={Tatsuo Kinashi and Nobuyuki Harada and Eva Severinson and Toshizumi Tanabe and Paschalis Sideras and Mikio Konishi and Chihiro Azuma and Akira Tominaga and Susanne Bergstedt‐Lindqvist and Masazumi Takahashi and Fumihiko Matsuda and Yoshio Yaoita and Kiyoshi Takatsu and Tasuku Honjo},
Proliferation and maturation of antigen-stimulated B cells are regulated by several soluble factors derived from macrophages and T cells1,2. These soluble factors are functionally divided into two groups: B-cell growth factor (BCGF), thought to be involved in B-cell proliferation; and B-cell differentiation factor (BCDF), responsible for maturation of activated B cells into immunoglobulin-secreting cells3–6. This classification needs to be re-examined in the light of the recent cloning of… 
The biology of interleukin-6.
Human IL-6 (BSF2) was originally identified as a factor in the culture supernatants of mitogen or antigen-stimulated peripheral mononuclear cells, which induced immunoglobulin production in Epstein Barr virus transformed B-cell lines or in Staphylococcus aureus Cowan 1 stimulated normal B cells.
Role of recombinant interleukin-1 compared to recombinant T-cell replacing factor/interleukin-5 in B-cell differentiation.
It is suggested that IL-1 acts primarily on resting B cells as a differentiation-inducing factor in the presence of antigen, and also acts as a 'priming' factor for TRF/IL-5 in 5-day cultures of B cells.
Molecular Biology of Interleukin 4 and Interleukin 5 Genes and Biology of their Products that Stimulate B Cells, T Cells and Hemopoietic Cells
A regulatory network formed between lymphoid cells and hemopoietic cells through the action of multiple lymphokines produced by activated T cells is revealed.
Revisiting the Identification and cDNA Cloning of T Cell-Replacing Factor/Interleukin-5
The history of the initial discovery of T cell-replacing factor/B cell growth factor II that appeared to be involved in B cell growth and differentiation and the discovery and characterization of interleukin-5 are summarized.
Interleukin 2 and low molecular weight B cell growth factor are T cell‐replacing factors for different subpopulations of human B cells*
Results show that high‐density B cells can in fact respond to TRF, and that IL2 and BCGFlow, act on different populations of B cells which may be defined by prior exposure to T cells.
B-Cell Growth and Differentiation Factors
  • K. Takatsu
  • Biology, Medicine
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1988
Evidence is provided that helper T cells recognize antigen in the context of class I1 major histocompatibility complex (MHC) molecules on accessory cells and/or B cells, and there is clear that the above two models represent two different mechanisms.


Factors affecting B-cell growth and differentiation.
  • T. Kishimoto
  • Biology, Medicine
    Annual review of immunology
  • 1985
In their experiment with murine splenic B cells, Kishimoto & Ishizaka showed that anti-SRBC response in T cell-depleted splenic lymphocytes could be reconstituted by culture supernatants of mixed lymphocyte reactions.
BCGFII activity on activated B cells of a purified murine T cell-replacing factor (TRF) from a T cell hybridoma (B151K12).
It is concluded that the TRF from B151K12 cells promotes growth of appropriately activated cells, such as DXS-stimulated normal cells and BCL1 tumor cells, suggesting that B151-TRF may act on B cells as B cell growth and differentiation factors.
Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities.
  • F. Lee, T. Yokota, C. Smith
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1986
It is demonstrated that a single cDNA clone distinct from interleukin 2 and interLEukin 3 encodes a polypeptide with multiple biological activities.
Purification and physicochemical characterization of murine T cell replacing factor (TRF).
The addition of appropriate amounts of N-acetylgalactosamine in the assay systems for TRF preferentially induced a profound suppression for TRf-mediated PFC responses, indicating that B151-TRF is a glycosylated glycoprotein containing N- acetylgalactsosamine residues.
Replacement of T-cell function by a T-cell product.
The existence of two distinct factors, one of which can completely replace T-cells, is reported, which is believed to be based on the heavy antigenic stimulation provided by the histocompatibility antigens carried by the B-cells.
Evidence for two distinct classes of murine B cell growth factors with activities in different functional assays
The results suggest that the two BCGF may have activity on two subsets of B cells that respond differentially to induction with the two polyclonal B cell activators, anti-Ig and DXS.
Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.
  • T. Yokota, F. Lee, K. Arai
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone, suggesting that MCGF is related to interleukin 3.
B Cell Growth and Differentiation Factors
Developing knowledge in this area is reviewed, finding cause for optimism and a hope that increased understanding of these factors and their mode of action will eventually reveal the basis of B cell immunoregulation.
Eosinophil differentiation factor also has B-cell growth factor activity: proposed name interleukin 4.
This paper reports that a mouse lymphokine that stimulates the production of functional eosinophils in liquid bone marrow cultures appears to be identical to the B-cell growth factor II described by Swain and Dutton, and proposes the name "interleukin 4" for this molecule, suggesting the defining property should be its eOSinophil-differentiating activity.
Secretion of IgG1 induction factor by T cell clones and hybridomas
New T cell lines secreting IgG1 induction factor are developed by cloning mixed lymphocyte culture populations and the assay is quantitative, reproducible and accurate, both when induction of IgGl as well as reduction of IgG3 and IgG2b were measured.