Cloning of colicin E1 tolerant tolC(mtcB) gene of Escherichia coli K12 and identification of its gene product

@article{Otsuji1982CloningOC,
  title={Cloning of colicin E1 tolerant tolC(mtcB) gene of Escherichia coli K12 and identification of its gene product},
  author={Nozomu Otsuji and Toshinori Soejima and Satoko Maki and Hideo Shinagawa},
  journal={Molecular and General Genetics MGG},
  year={1982},
  volume={187},
  pages={30-36}
}
A mutation in the tolC(mtcB) gene of Escherichia coli K12 results in increased sensitivity to sodium dodecylsulfate (SDS), sodium deoxycholate, basic dyes, mitomycin C, and bleomycin, and makes the cell tolerant to the killing action of colicin E1. From lysogens with λcI857S7 integrated at a secondary attachment site, a transducing phage (λdtolC +) that transduces a tolC recipient to SDS resistance was isolated. A recombinant DNA molecule was constructed in vitro from plasmid pBR322 as a vector… CONTINUE READING
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