Cloning of chicken anemia virus vp3 gene and apoptosis inductive effect of vp3 genein vitro

Abstract

Using PCR technique, the vp3 gene of chicken anemia virus (CAV) was cloned into the eukaryotic expression vector pcDNA3 to construct a recombinant pcDNA-vp3. Restriction enzyme digestion and sequencing analysis revealed that CAV vp3 gene was correctly inserted into the blank vector pcDNA3. After LipofectAMINETM-mediated transfectionin vitro with pcDNA-vp3… (More)
DOI: 10.1007/BF02829409

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