Cloning of cell-specific secreted and surface proteins by subtractive antibody screening

@article{Scherer1998CloningOC,
  title={Cloning of cell-specific secreted and surface proteins by subtractive antibody screening},
  author={Phillip E Scherer and Perry E Bickel and Mariana Kotler and Harvey F. Lodish},
  journal={Nature Biotechnology},
  year={1998},
  volume={16},
  pages={581-586}
}
To identify and clone genes that encode cell- or tissue-specific secreted and surface proteins, a polyclonal antiserum was raised against a complex mixture of surface or secreted proteins from the target cell, followed by immunodepletion of antibodies that recognize proteins from a nontarget cell or tissue. The depleted antiserum is used to screen bacteriophage cDNA expression libraries. Because of our interest in how adipocytes communicate with other cells, we have used this method to clone… CONTINUE READING

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