Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter

  title={Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter},
  author={Yoshihiko Noma and Paschalis Sideras and Takayuki Naito and Susanne Bergstedt-Lindquist and Chihiro Azuma and Eva Severinson and Toshizumi Tanabe and Tatsuo Kinashi and Fumihiko Matsuda and Yoshio Yaoita and Tasuku Honjo},
Complementary DNA encoding the IgG1 induction factor, the first lymphokine directed to B lymphocytes, from a murine T-cell line has been cloned using a new strategy. The putative primary amino-acid sequence was deduced from the nucleotide sequence determined. The lymphokine synthesized by the direction of this cloned cDNA has many other functions, such as production of B-cell growth factor-1 and induction of Ia on B cells. 
Molecular cloning and expression of a human B-cell growth factor gene in Escherichia coli.
A clone was isolated that contained the proper structural sequence to encode biologically active, 12-kilodalton BCGF in Escherichia coli and to hybridize to a specific messenger RNA, identified by in vitro translation in Xenopus laevis oocytes.
Structure and Function of Interleukins 4 and 5
Since the recombinant factors were also shown to affect bone marrow‐derived cells other than B cells, the names of interleukins 4 and 5 were proposed for IgG1 induction factor and T cell replacing factor, respectively.
Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus.
The 5'-upsteam region of the genomic gene and cDNA encoding interleukin 4 (IL-4) has been isolated and sequenced from the inbred mouse strain MSKR derived from a Japanese wild mouse using polymerase
The isolation interleukin 4 and sequence of sheep
The nucleotide and amino acid sequences of a sheep 11-4 cDNA are presented and compared with sequences from other species and shared 73 and 62% homology with human and mouse cDNA homologues, respectively.
The isolation and sequence of sheep interleukin 4.
The nucleotide and amino acid sequences of a sheep IL-4 cDNA are presented and compared with sequences from other species, finding that the sheep cDNA shared 73 and 62% homology with human and mouse cDNA homologues, respectively.
Interleukin 4 (IgG1 induction factor): a multifunctional lymphokine acting also on T cells
Rec recombinant IL4 activated the murine T cell line CTLL to increased DNA synthesis but not to growth but also activated normal concanavalin A (Con A)‐stimulated T cells both to increasedDNA synthesis and to growth.
Revisiting the Identification and cDNA Cloning of T Cell-Replacing Factor/Interleukin-5
The history of the initial discovery of T cell-replacing factor/B cell growth factor II that appeared to be involved in B cell growth and differentiation and the discovery and characterization of interleukin-5 are summarized.
A new interleukin with pleiotropic activities
  • T. Kishimoto, T. Hirano
  • Biology, Medicine
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1988
Molecular cloning of the cDNA has revealed that BSF2 is identical with 26 kD protein, interferon β2, plasmacytoma growth factor and hepatocyte stimulating factor and the designation “IL‐6” has been proposed for this molecule.
Lymphotoxin is an important T cell-derived growth factor for human B cells.
Recombinant lymphotoxin enhanced the proliferation of activated B cells and augmented B cell proliferation and immunoglobulin secretion induced by interleukin-2.


Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.
  • T. Yokota, F. Lee, K. Arai
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone, suggesting that MCGF is related to interleukin 3.
Cloning and expression of murine immune interferon cDNA.
  • P. Gray, D. Goeddel
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1983
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line and expressed in Escherichia coli under trp promoter control.
Secretion of IgG1 induction factor by T cell clones and hybridomas
New T cell lines secreting IgG1 induction factor are developed by cloning mixed lymphocyte culture populations and the assay is quantitative, reproducible and accurate, both when induction of IgGl as well as reduction of IgG3 and IgG2b were measured.
Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells.
Results demonstrate that identification of full-length cDNA clones for many lymphokines may be achieved entirely on the basis of detection of the functional polypeptides in mammalian cells.
Rat insulin genes: construction of plasmids containing the coding sequences.
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the
Structure and expression of the mRNA for murine granulocyte‐macrophage colony stimulating factor.
A cDNA containing a virtually complete copy of the mRNA for the haemopoietic growth regulator, granulocyte‐macrophage colony stimulating factor, has been isolated from a murine T lymphocyte cDNA library and expression studies indicated that the second AUG is able to initiate the translation and secretion of GM‐CSF.
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
The method of mRNA synthesis involves in vitro transcription of cDNAs which have been cloned into SP6 vectors and enables one to produce large amounts of mRNA and consequently protein from any cDNA clone.
Immunoglobulin C gene expression. IV. Alternative control of IgG1‐producing cells by helper cell‐derived B cell‐specific growth or maturation factors
The results demonstrate that helper cells regulate IgG1 expression by two alternative modes: growth‐promoting factors expand membrane IgG 1‐bearing nonsecretory activated B cells, while maturation factors induce terminal differentiation to high‐rate secretion of IgG2 in previously switched cells.
Identification of a T cell-derived b cell growth factor distinct from interleukin 2
A factor found in induced supernatants of the mouse thymoma EL4 that co-stimulates with anti-IgM antibodies in short-term cultures of purified B lymphocytes to induce polyclonal B cell proliferation but not antibody-forming cell production is reported.
Functional and non-functional joining in immunoglobulin light chain genes of a mouse myeloma
Leader, variable (V) and joining (J) gene segments, and adjacent regions of two rearranged alleles of the same κ-chain producing mouse myeloma, comprising approximately 3,200 base pairs, have been