Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli.

Abstract

cDNA clones of the mRNA coding for tissue-type plasminogen activator (t-PA) have been obtained and their nucleotide sequences compared to those reported previously. A gene coding for t-PA has been reconstructed and inserted into vectors for expression in prokaryotic cells. Relatively high levels of t-PA accumulated in inclusion bodies in Escherichia coli… (More)

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@article{Harris1986CloningOC, title={Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli.}, author={T. J. Harris and T. Patel and Felicia Marston and Susan J. Little and J. S. Emtage and Ghislain Opdenakker and G. Volckaert and Wilfried Rombauts and An D. Billiau and Pieter De Somer}, journal={Molecular biology & medicine}, year={1986}, volume={3 3}, pages={279-92} }