Cloning of alpha 2 chain of type VI collagen and expression during mouse development.

@article{Ibrahimi1993CloningOA,
  title={Cloning of alpha 2 chain of type VI collagen and expression during mouse development.},
  author={Azeddine Ibrahimi and B K Bertrand and Sylvie Bardon and Ez Z. Amri and Paola Grimaldi and G{\'e}rard Ailhaud and Christian Dani},
  journal={The Biochemical journal},
  year={1993},
  volume={289 ( Pt 1)},
  pages={141-7}
}
We have previously described the molecular cloning of a cDNA probe which detects a 6 kb mRNA termed pOb24. pOb24 mRNA appeared to be a marker of the preadipose state both in vitro and in vivo. A pOb24 genomic fragment was isolated and used to screen cDNA libraries in order to isolate the full-length pOb24 cDNA and to identify the corresponding protein. The screening yielded a new cDNA clone which detected a 3.7 kb mRNA species in addition to the 6 kb mRNA species. Sequences at the 3' end of the… CONTINUE READING