Cloning of agarase gene from non-marine agarolytic bacterium Cellvibrio sp.

@article{Ariga2012CloningOA,
  title={Cloning of agarase gene from non-marine agarolytic bacterium Cellvibrio sp.},
  author={Osamu Ariga and Takayoshi Inoue and Hajime Kubo and Kimi Minami and Mitsuteru Nakamura and Michi Iwai and Hironori Moriyama and Mitsunori Yanagisawa and Kiyohiko Nakasaki},
  journal={Journal of microbiology and biotechnology},
  year={2012},
  volume={22 9},
  pages={
          1237-44
        }
}
Agarase genes of non-marine agarolytic bacterium Cellvibrio sp. were cloned into Escherichia coli and one of the genes obtained using HindIII was sequenced. From nucleotide and putative amino acid sequences (713 aa, molecular mass; 78,771 Da) of the gene, designated as agarase AgaA, the gene was found to have closest homology to the Saccharophagus degradans (formerly, Microbulbifer degradans) 2-40 aga86 gene, belonging to glycoside hydrolase family 86 (GH86). The putative protein appears to be… 
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