Cloning of a putative monogalactosyldiacylglycerol synthase gene from rice (Oryza sativa L.) plants and its expression in response to submergence and other stresses

@article{Qi2004CloningOA,
  title={Cloning of a putative monogalactosyldiacylglycerol synthase gene from rice (Oryza sativa L.) plants and its expression in response to submergence and other stresses},
  author={YanHua Qi and Yasuo Yamauchi and Jianqun Ling and Naoyoshi Kawano and Debao Li and Kiyoshi Tanaka},
  journal={Planta},
  year={2004},
  volume={219},
  pages={450-458}
}
Suppression subtractive hybridization was used to construct a subtractive cDNA library from plants of non-submerged and 7-day-submerged rice (Oryza sativa L., FR13A, a submergence-tolerant cultivar). One clone of the subtractive cDNA library, S23, was expressed abundantly during submergence. The full length of S23 was amplified using 5′- and 3′-rapid amplification of cDNA ends, and found to consist of 1,671 bp with an open reading frame of 1,077 bp (181–1257) encoding 358 amino acids. Its… CONTINUE READING
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