Cloning of a novel phospholipase A2 from the cnidarian Adamsia carciniopados.

@article{Talvinen2002CloningOA,
  title={Cloning of a novel phospholipase A2 from the cnidarian Adamsia carciniopados.},
  author={Kati A. Talvinen and Timo J. Nevalainen},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2002},
  volume={132 3},
  pages={571-8}
}
PLA2 catalytic activity was detected in homogenised tissues, including tentacles and acontia (structures for preying and defence, respectively), of the sea anemone Adamsia carciniopados. Nested reverse transcription polymerase chain reaction (RT PCR) with degenerate primers and rapid amplification of cDNA ends (RACE) were used to clone a novel phospholipase A2 from Adamsia carciniopados (AcPLA2). AcPLA2 contains a putative prepropeptide of 37 residues, ending with a basic doublet followed by a… CONTINUE READING