Cloning of a new cytokine that induces IFN-γ production by T cells

  title={Cloning of a new cytokine that induces IFN-$\gamma$ production by T cells},
  author={Haruki Okamura and Hiroko Tsutsui and Toshinori Komatsu and Masuo Yutsudo and Akira Hakura and Tadao Tanimoto and Kakuji Torigoe and Takanori Okura and Yoshiyuki Nukada and K Hattori and Kenji Akita and Motoshi Namba and Fujimi Tanabe and K Konishi and Shigeharu Okayama-shi Fukuda and Masashi Kurimoto},
THE mechanism underlying the differentiation of CD4+ T cells into functionally distinct subsets (Thl and Th2) is incompletely understood1–3, and hitherto unidentified cytokines may be required for the functional maturation of these cells4. Here we report the cloning of a recently identified IFN-γ-inducing factor (IGIF) that augments natural killer (NK) activity in spleen cells5,6. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids, which have no… 
IL-18 and IL-18 Receptor Knockout Mice
IL-18 synergizes with IL-12 for IFN-γ production by a wide range of cell types, including NK cells, and B-cells, macrophages, dendritic Cells, and smooth muscle cells (9,12–15).
Caspase-1 processes IFN-γ-inducing factor and regulates LPS-induced IFN- γ production
The results implicate caspase-1 in the physiological production of IGIF and demonstrate that it plays a critical role in the regulation of multiple proinflammatory cytokines, which would provide a new class of anti-inflammatory drugs with multipotent action.
Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ
It is indicated that ChIL-18 and ChIFN-γ could inhibit IBDV infection and the combination of ChIL/ChIFN/PET28a has a better inhibitory effect than either cytokine alone.
Interleukin 18
  • S. Swain
  • Biology
    The Journal of experimental medicine
  • 2001
IL-18 is expressed at sites of chronic inflammation, in autoimmune diseases, in a variety of cancers, and in the context of numerous infectious diseases, and its potential effector and regulatory role in several human disease states including autoimmunity and infection is addressed.
Interleukin 12-dependent interferon gamma production by CD8alpha+ lymphoid dendritic cells.
The data indicated that dendritic cells are potent in producing IFN-γ in response to IL-12 produced by bacterial infection and play an important role in innate immunity and subsequent T helper cell type 1 development in vivo.
Cloning and characterization of a new isoform of mouse interleukin-18.
IL-18s could enhance the ability of IL-18 to increase IFN-lambda production by approximately 40% in mouse splenocytes, suggesting that IL- 18s might regulate the activity of IL -18 in the physiological conditions.
Cloning of a cDNA Encoding Bovine Interleukin-18 and Analysis of IL-18 Expression in Macrophages and Its IFN-γ-Inducing Activity
This work isolated and sequenced a cDNA encoding bovine IL-18, a recently described cytokine that enhances interferon-γ (IFN-γ) production, either independently or synergistically with IL-12, and found it to be pivotal in host defense against intracellular pathogens.
Mycobacterium bovis BCG Producing Interleukin-18 Increases Antigen-Specific Gamma Interferon Production in Mice
Results indicate that the production of IL-18 by recombinant BCG may enhance the immunomodulatory properties of BCG further toward a Th1 profile, which may be particularly useful for immunotherapeutic or prophylactic interventions in which a Th2 response is most desirable.
In vivo immunomodulation following intradermal injection with DNA encoding IL-18.
Injection of DNA encoding IL-18 into the skin modulates both Ag-specific humoral and T cell responses upon mycobacterial infection, which increases the Th1 type response, which may be particularly useful for the development of new immunotherapeutic or immunoprotective approaches against infections by intracellular parasites.


Purification of a factor which provides a costimulatory signal for gamma interferon production
These studies confirm the existence of a soluble protein factor which is able to exhibit a novel accessory function in IFN-gamma production in resting T or natural killer cells.
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Endogenous interleukin 12 (IL-12) regulates granuloma formation induced by eggs of Schistosoma mansoni and exogenous IL-12 both inhibits and prophylactically immunizes against egg pathology
The latter findings suggest that simultaneous vaccination with antigen plus IL-12 may provide a strategy for the prevention of schistosome egg pathology as well as other diseases stemming from Th2 cytokine production.
Autocrine growth and tumorigenicity of interleukin 2-dependent helper T cells transfected with IL-2 gene
Evidence is provided that the aberrant activation of the IL-2 autocrine circuit can lead T cells to malignant transformation.
Generation of interleukin 4 (IL-4)-producing cells in vivo and in vitro: IL-2 and IL-4 are required for in vitro generation of IL-4- producing cells
T cell populations derived from naive mice produce very small amounts of interleukin 4 in response to stimulation on anti-CD3-coated dishes, and it is possible that a portion of the IL-4-producing activity of T cells from activated donors is IL-2 independent.
Interleukin‐12 is required for interferon‐γ production and lethality in lipopolysaccharide‐induced shock in mice
IL‐12 is required for IFN‐γ production and lethality in an endotoxic shock model in mice because co‐injection of TNF‐α and IL‐12, sufficient to induce serum concentrations of both cytokines higher and more persistent than those obtained by injection of LPS, was not sufficient to induced IFN-γ production in vivo.
Importance of direct hepatocytolysis by liver macrophages in experimental fulminant hepatitis.
The results suggest that the liver adherent cells accumulated and partly stimulated by P. acnes-treatment might rapidly lyse the autologous hepatocytes once triggered by lipopolysaccharide and that the TNF-alpha these liver adherent Cells produced might upregulate their own hepatocytotoxic ability.
Interleukin 12 in host defense against microbial pathogens.
  • R. Locksley
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1993
The role of a relatively recently described cytokine, interleukin 12 (IL-12), in the subsequent cognate immune response orchestrated by T cells is considered.