Cloning of a human gene encoding the general transcription initiation factor IIB

@article{Ha1991CloningOA,
  title={Cloning of a human gene encoding the general transcription initiation factor IIB},
  author={Ilho Ha and William S. Lane and Danny F Reinberg},
  journal={Nature},
  year={1991},
  volume={352},
  pages={689-695}
}
Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic σ-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.