Cloning of a higher-plant plastid omega-6 fatty acid desaturase cDNA and its expression in a cyanobacterium.

@article{Hitz1994CloningOA,
  title={Cloning of a higher-plant plastid omega-6 fatty acid desaturase cDNA and its expression in a cyanobacterium.},
  author={William D. Hitz and Thomas J S Carlson and J. Richard Booth and Anthony J. Kinney and Kevin L Stecca and N. Sudhakar Yadav},
  journal={Plant physiology},
  year={1994},
  volume={105 2},
  pages={635-41}
}
Oligomers based on amino acids conserved between known plant omega-3 and cyanobacterium omega-6 fatty acid desaturases were used to screen an Arabidopsis cDNA library for related sequences. An identified clone encoding a novel desaturase-like polypeptide was used to isolate its homologs from Glycine max and Brassica napus. The plant deduced amino acid sequences showed less than 27% similarity to known plant omega-6 and omega-3 desaturases but more than 48% similarity to cyanobacterial omega-6… CONTINUE READING

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