Cloning of a gene encoding thermostable cellobiohydrolase from Thermoascus aurantiacus and its expression in yeast

@article{Hong2003CloningOA,
  title={Cloning of a gene encoding thermostable cellobiohydrolase from Thermoascus aurantiacus and its expression in yeast},
  author={Jiong Hong and Hisanori Tamaki and Kenji Yamamoto and Hidehiko Kumagai},
  journal={Applied Microbiology and Biotechnology},
  year={2003},
  volume={63},
  pages={42-50}
}
A gene encoding a cellobiohydrolase (CBH) was isolated from Thermoascus aurantiacus IFO 9748 and designated as cbh1. The deduced amino acid sequence encoded by cbh1 showed high homology with the sequence of glycoside hydrolase family 7. To confirm the sequence of the gene encoding the CBH, the cloned gene was expressed in the yeast Saccharomyces cerevisiae, in which no cellulase activity was found, and the gene product was purified and subjected to enzymatic characterization. The recombinant… CONTINUE READING
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Purification and properties of the cellulases from the thermophilic fungus Thermoascus aurantaicus

  • C Tong, LC Anthony, GS Maxwell
  • Biochem J
  • 1980
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