Cloning of a functional human adenine phosphoribosyltransferase (APRT) gene: identification of a restriction fragment length polymorphism and preliminary analysis of DNAs from APRT-deficient families and cell mutants.

@article{Stambrook1984CloningOA,
  title={Cloning of a functional human adenine phosphoribosyltransferase (APRT) gene: identification of a restriction fragment length polymorphism and preliminary analysis of DNAs from APRT-deficient families and cell mutants.},
  author={Peter J. Stambrook and M K Dush and John J. Trill and Jay A. Tischfield},
  journal={Somatic cell and molecular genetics},
  year={1984},
  volume={10 4},
  pages={
          359-67
        }
}
A complete human APRT gene has been isolated from a lambda phage genomic library using cloned mouse APRT DNA as a probe. The human gene, contained in a recombinant lambda phage designated lambda Huap15, is functional by virtue of its capacity to transfer human APRT activity to Aprt- mouse recipient cells after phage-mediated transfection. Digestion of lambda Huap15 DNA with BamH1 generated a 2.2-kb fragment that is the only fragment of eight produced to hybridize with the mouse APRT gene. This… CONTINUE READING