Cloning of TTG1 gene and PCR identification of genomes A, B and C in Brassica species

@article{Yan2014CloningOT,
  title={Cloning of TTG1 gene and PCR identification of genomes A, B and C in Brassica species},
  author={Mingli Yan and Xianjun Liu and Chunyun Guan and Lili Liu and Jianhua Xiang and Ying Lu and Zhongsong Liu},
  journal={Genetica},
  year={2014},
  volume={142},
  pages={169-176}
}
Arabidopsis Transparent Testa Glabra 1 (TTG1) genes were cloned from three diploid Brassica species (B. rapa, B. nigra and B. oleracea) and two amphidiploids species (B. juncea and B. carinata) by homology cloning. TTG1 homologues identified in all the accessions of the investigated species had a coding sequence of 1,014 bp. One copy was obtained from each diploid species and two copies from each amphidiploid species. Combined analysis of the TTG1 sequences cloned in this study with those… CONTINUE READING