Cloning of Small RNA Molecules

@article{Pfeffer2005CloningOS,
  title={Cloning of Small RNA Molecules},
  author={S{\'e}bastien Pfeffer and Mariana Lagos-Quintana and Thomas Tuschl},
  journal={Current Protocols in Molecular Biology},
  year={2005},
  volume={72}
}
Small RNAs that are derived from dsRNA precursors act as guide RNAs during sequence‐specific epigenetic regulation of eukaryotic gene expression. These small regulatory RNAs are between 20 and 30 nucleotides in length, and fall into one or more of the following categories: small interfering RNAs (siRNAs), microRNAs (miRNAs), and heterochromatic siRNAs (hsiRNAs). Procedures to record the profile of small RNAs expressed in cultured cells or tissues are described. The small RNAs are directionally… 
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