Cloning of Prevotella intermedia loci demonstrating multiple hemolytic domains.

Abstract

A gene bank was created from Prevotella intermedia strain 27 chromosomal DNA, and a clone was isolated that conferred the expression of two separate modes of hemolytic activity in recombinant Escherichia coli. The original recombinant hemolytic strain (EB34) contained plasmid, pEB34, with a 5.6-kb insert from Sau 3 AI-digested P. intermedia strain 27… (More)

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Cite this paper

@article{Beem1999CloningOP, title={Cloning of Prevotella intermedia loci demonstrating multiple hemolytic domains.}, author={J Elaine Beem and W E Nesbitt and K P Leung}, journal={Oral microbiology and immunology}, year={1999}, volume={14 3}, pages={143-52} }