Cloning of Clostridium thermocellum endoglucanase genes in Escherichia coli.


Six independent and distinct cel genes coding endoglucanases have been selected from C. thermocellum pUC19-based gene bank in E. coli TG1. E. coli-derived Cel-proteins possessing Mr from 39,000 to 61,000 are able to cleave lichenan, as well as xylan and carboxymethyl cellulose. Cel 7- and Cel 8-endoglucanases are characterized by cellobiohydrolase type substrate specificity, being able to cleave model fluorogenic aryldisaccharide substrate MU-G2. The clone pCU110 (cel 7) produces about 10-fold more endoglucanase activity than other clones.


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@article{Bumazkin1990CloningOC, title={Cloning of Clostridium thermocellum endoglucanase genes in Escherichia coli.}, author={B K Bumazkin and Galina A Velikodvorskaya and K Tuka and M A Mogutov and Strongin AYa}, journal={Biochemical and biophysical research communications}, year={1990}, volume={167 3}, pages={1057-64} }