Cloning of African trypanosomes in mice immunosuppressed by cyclophosphamide treatment.

@article{Smith1982CloningOA,
  title={Cloning of African trypanosomes in mice immunosuppressed by cyclophosphamide treatment.},
  author={Christopher W. J. Smith and Richard F. Levine and John M. Mansfield},
  journal={The American journal of tropical medicine and hygiene},
  year={1982},
  volume={31 6},
  pages={1098-102}
}
The use of cyclophosphamide-treated mice for purpose of cloning the African trypanosomes was assessed. C3HeB/FeJ mice were injected with 200 mg/kg cyclophosphamide (CY) 24-72 hours prior to infection with a single trypanosome isolated from Trypanosoma rhodesiense infected blood samples. All CY-treated mice exhibited depressed parasite-exposure, which was a period of time sufficient to grow trypanosomes from a single organism to a fulminating parasitemia. Cloning efficiency was routinely 45% in… CONTINUE READING

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