Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing d-xylose fermentation by recombinant Saccharomyces cerevisiae

@article{Sales2015CloningNS,
  title={Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing d-xylose fermentation by recombinant Saccharomyces cerevisiae},
  author={Belisa B. de Sales and Bruna Scheid and Davi Ludvig Gonçalves and Marilia M. Knychala and Akinori Matsushika and Elba P. S. Bon and Boris Ugarte Stambuk},
  journal={Biotechnology Letters},
  year={2015},
  volume={37},
  pages={1973-1982}
}
Since uptake of xylose limits its fermentation, we aimed to identify novel sugar transporters from Scheffersomyces stipitis that allow xylose uptake and fermentation by engineered Saccharomyces cerevisiae. An hxt-null S. cerevisiae strain, lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ) but having high xylose reductase, xylitol dehydrogenase… CONTINUE READING