Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR

@inproceedings{Kim2010CloningLN,
  title={Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR},
  author={Jeffrey H. Kim and Zhiyang Feng and John D. Bauer and Dimitris Kallifidas and Paula Y. Calle and Sean F. Brady},
  booktitle={Biopolymers},
  year={2010}
}
A single gram of soil can contain thousands of unique bacterial species, of which only a small fraction is regularly cultured in the laboratory. Although the fermentation of cultured microorganisms has provided access to numerous bioactive secondary metabolites, with these same methods it is not possible to characterize the natural products encoded by the uncultured majority. The heterologous expression of biosynthetic gene clusters cloned from DNA extracted directly from environmental samples… CONTINUE READING
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