Cloning gibberellin dioxygenase genes from pumpkin endosperm by heterologous expression of enzyme activities in Escherichia coli.

@article{Lange1997CloningGD,
  title={Cloning gibberellin dioxygenase genes from pumpkin endosperm by heterologous expression of enzyme activities in Escherichia coli.},
  author={Theo Lange},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 12},
  pages={6553-8}
}
  • Theo Lange
  • Published 1997 in
    Proceedings of the National Academy of Sciences…
Gibberellin (GA) plant hormones are biosynthesized via complex pathways, the final steps of which are catalyzed by 2-oxoglutarate-dependent dioxygenases. Here, the cloning of two such enzymes, the GA 7-oxidase and the GA 20-oxidase, is reported using a novel approach, namely, by screening for GA dioxygenase activities expressed as T7 gene 10 fusion proteins in recombinant Escherichia coli. In vitro translation products of mRNA from endosperm of immature pumpkin seeds contained three GA… CONTINUE READING
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