Cloning differentially expressed genes by linker capture subtraction.

  title={Cloning differentially expressed genes by linker capture subtraction.},
  author={Ming Yang and Arthur J. Sytkowski},
  journal={Analytical biochemistry},
  volume={237 1},
We have developed a simple and effective method, designated linker capture subtraction (LCS), for cloning differentially expressed genes between two cell types or between cells treated in two different ways. In the first step of the method, two mRNA populations are converted to double-stranded cDNAs, fragmented, and ligated to linkers for PCR amplification. In the second step, the linkered DNA (tester) from one mRNA population is hybridized to an excess of the unlinkered DNA (driver) from the… CONTINUE READING

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