Cloning, characterization and promoter analysis of common carphairy/Enhancer-of-split-related gene,her6

Abstract

Some members ofhairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes, theher6 gene, a member of the HES family, is the homologue ofhes1 in mammals and chicken. In this study, theher6 gene and its full-length cDNA from the common carp (Cyprinus carpio) were isolated and characterized. The genomic sequence of common carpher6 is approximately 1.7 kb, with four exons and three introns, and the full-length cDNA of 1314 bp encodes a putative polypeptide of 271 amino acids. To analyse the promoter sequence of common carpher6, sequences of various lengths upstream from the transcription initiation site ofher6 were fused to enhanced green fluorescent protein gene (eGFP) and introduced into zebrafish embryos by microinjection to generate transgenic embryos. Our results show that the upstream sequence of 500 bp can direct highly efficient and tissue-specific expression ofeGFP in zebrafish embryos, whereas a fragment of 200 bp containing the TATA box and a partial suppressor of hairless paired site sequence (SPS) is not sufficient to driveeGFP expression in zebrafish embryos.

DOI: 10.1007/BF02935327

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Cite this paper

@article{Liu2006CloningCA, title={Cloning, characterization and promoter analysis of common carphairy/Enhancer-of-split-related gene,her6}, author={Jing Liu and Yong-hua Sun and Na Wang and Ya-Ping Wang and Zuoyan Zhu}, journal={Journal of Genetics}, year={2006}, volume={85}, pages={171-178} }