Cloning and transcriptional control of a eucaryotic permease gene.

  title={Cloning and transcriptional control of a eucaryotic permease gene.},
  author={M. R. Chevallier},
  journal={Molecular and cellular biology},
  volume={2 8},
The uracil permease gene of the yeast Saccharomyces cerevisiae was cloned on a hybrid plasmid which replicates autonomously in both yeast and Escherichia coli. Cloning was carried out by complementation in yeast. The smallest DNA fragment found to complement the uracil permease deficiency in recipient yeast cells measured approximately 2.3 kilobases. In strains transformed by the plasmid with the uracil permease gene inserted, initial rates of uracil uptake increased up to 25 times more than… CONTINUE READING


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