Cloning and tissue-specific expression of a delta-COP homologue in a freshwater and a brackish water-adapted strain of rainbow trout (Oncorhynchus mykiss).

Abstract

In eukaryotic cells, intracellular transport is mediated by coated vesicular carriers. Coat proteins I (COPI) vesicles are involved in the retrograde transport from Golgi apparatus to the endoplasmic reticulum. The COPI complex is composed of ADP-ribosylation factor 1 and coatomer comprising seven subunits, termed alpha-zeta. We isolated and characterised a cDNA sequence from rainbow trout homolog to delta-subunit of COPI complex (delta-COP). Trout delta-COP gene encodes a protein of 509 aa including a characteristic Mu homology domain. Searches at the Ensemble Genome browser identified three additional teleostean delta-COP-like sequences from pufferfish, rice fish, and stickleback. Sequence identity of piscine delta-COP protein sequences is greater than 84%. Moreover, a phylogenetic analysis indicates that delta-COP protein sequences are strongly conserved among vertebrate species. delta-COP homologue is ubiquitously expressed in trout tissues. Quantitative Real-Time RT-PCR revealed that delta-COP is differentially expressed in liver and gill tissue of two rainbow trout strains, the freshwater strain STEELHEAD and the brackish water-adapted strain BORN.

Statistics

01002002011201220132014201520162017
Citations per Year

88 Citations

Semantic Scholar estimates that this publication has 88 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Rebl2009CloningAT, title={Cloning and tissue-specific expression of a delta-COP homologue in a freshwater and a brackish water-adapted strain of rainbow trout (Oncorhynchus mykiss).}, author={Alexander Rebl and E. Margot Anders and Klaus Wimmers and Tom Goldammer}, journal={Genes & genetic systems}, year={2009}, volume={84 3}, pages={239-43} }