Cloning and substrate specificity of a human phenol UDP-glucuronosyltransferase expressed in COS-7 cells.

@article{Harding1988CloningAS,
  title={Cloning and substrate specificity of a human phenol UDP-glucuronosyltransferase expressed in COS-7 cells.},
  author={D M Harding and Sylvie Fournel-Gigleux and Mandy Jackson and Brian Burchell},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1988},
  volume={85 22},
  pages={8381-5}
}
A rat kidney phenol UDP-glucuronosyltransferase cDNA was used to isolate a human liver phenol UDP-glucuronosyltransferase cDNA by screening of a human liver cDNA library in the expression vector lambda gt11. The 2.4-kilobase cDNA contained an open reading frame of 1593 base pairs coding for a protein of 531 residues. The human liver cDNA was subcloned into the vector pKCRH2. Transfection of this recombinant plasmid into COS-7 cells allowed the expression of a protein of approximately 55 kDa… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 41 extracted citations

Similar Papers

Loading similar papers…