Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

@article{Shizuya1992CloningAS,
  title={Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.},
  author={Hiroaki Shizuya and Bruce W. Birren and Ung Jin Kim and Valeria Mancino and T N Slepak and Y. Tachiiri and Melvin I. Simon},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 18},
  pages={8794-7}
}
A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency… CONTINUE READING
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