Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus.

Abstract

The mRNA transcribed from the N gene of viral haemorrhagic septicaemia virus (VHSV) of salmonids has been cloned in Escherichia coli and expressed. Fusion proteins were recognized by monoclonal antibody directed against the N protein from the viral particle. A 1212 bp long open reading frame (ORF) coding for 404 amino acids with a calculated Mr of 44590 was… (More)

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@article{Bernard1990CloningAS, title={Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus.}, author={Julie L Bernard and F Lecocq-Xhonneux and Martina Rossius and Marc Thiry and P de Kinkelin}, journal={The Journal of general virology}, year={1990}, volume={71 ( Pt 8)}, pages={1669-74} }