Cloning and sequencing of yajC and secD homologs of Brucella abortus and demonstration of immune responses to YajC in mice vaccinated with B. abortus RB51.

@article{Vemulapalli1998CloningAS,
  title={Cloning and sequencing of yajC and secD homologs of Brucella abortus and demonstration of immune responses to YajC in mice vaccinated with B. abortus RB51.},
  author={Ramesh Vemulapalli and Alison J Duncan and Stephen M. Boyle and Nammalwar Sriranganathan and Thomas E Toth and Gerhardt G. Schurig},
  journal={Infection and immunity},
  year={1998},
  volume={66 12},
  pages={5684-91}
}
To identify Brucella antigens that are potentially involved in stimulating a protective cell-mediated immune response, a gene library of Brucella abortus 2308 was screened for the expression of antigens reacting with immunoglobulin G2a antibodies from BALB/c mice vaccinated with B. abortus RB51. One selected positive clone (clone MCB68) contained an insert of 2.6 kb; nucleotide sequence analysis of this insert revealed two open reading frames (ORFs). The deduced amino acid sequences of the… CONTINUE READING

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